Okay, I'm really sorry if someone already asked this, but seeing all these asks on my dash got me all confused and I just spent hours reading and researching the homestuck wiki, but I'm still really really confused on one thing. So the pre-scratch trolls, (with meenah, and kanri and stuff) created the beta trolls (with karkat and co.) and karkat created the ancestors (the sufferer, and co.)???? But in chronological terms, the ancestors came first, then pre-scratch, then post-scratch??

Nope, the pre-scratch trolls didn’t do any ectobiology.  Karkat made everyone.  And no, the Ancestors lived in the post-scratch universe.