So from that talk of raising mother grubs in the last upd8, while albeit short, does this or does this not mean that the Matriorb was not destroyed by eridan in this timeline? I mean that I do know Kanaya was still killed by him (still came back though) but I am unsure as to whether or not talk of raising one means that they still have the Matriorb?

The matriorb was still destroyed.  If Roxy practices some more she’ll have the ability to make another one though.