Even if the Frog is physical, I doubt neutering it would do anything since universe creation doesn't rely on Frog gonads at all. You'd probably just end up partitioning that universe into a couple of disconnected pieces, where you can't travel through physical space from one to another anymore.

I mean, physically it doesn’t work like that, but thematically it may work. I mean let’s be fair, it’s not gonads, but the entirety of SBURB’s last steps has the Genesis Tadpole as the Sperm Cell shot from the Volcano and into Skaia as the Egg Cell, fertilization has NEVER been more graphic yet symbolic at the same time! Sooo, thematically, yeah.