Like Pa Harley, who had Joey and Jude, is it possible for the troll ancestors to have descendants aside from their dancestors?

I don’t see why not, in fact it’s very likely- Like, they all reached Adulthoods. If we know something about Troll Culture is that they’re REQUIRED to provide their genetic material when the time comes or they will be culled. So outside of the Signless, and possibly the Handmaid, it’s almost a certainty every single other Ancestor fucked at some point.